Plasmid

Part:BBa_K4455005:Design

Designed by: Gabriella Cerna   Group: iGEM22_ASU   (2022-10-11)


FMDV 2A Peptide Optimized for Chlamydomonas Reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This 2A peptide was codon optimized for usage in Chlamydomonas reinhardtii.



Source

The sequence for FMDV originates from Foot and Mouth Disease Virus. While the sequence for the 2A peptide itself originates from a virus, it only functions to enable the production of independent proteins when inserted between the coding sequences of proteins of interest.

References